Efektivitas Ekstrak Teh Hijau (Camellia sinensis L.) terhadap Penurunan Kadar Glukosa Darah Puasa Tikus Wistar (Rattus Norvegicus) yang di Induksi Aloksan
Keywords:
Alloxan; FBG Level; Green Tea; Hyperglycemia
Abstract
The sufferers of Diabetes Mellitus (DM) in Indonesia is the highest with fourth number in the world, with a mortality rate of 1.2 million in 2012. Because of it, DM research using antihyperglycemic green tea is needed. This study aims to determine the effectiveness of green tea extracts on decreasing fasting blood glucose (KGDP) levels in alloxan-induced white male Wistar rats. Samples were 30 male rats, aged 8-12 weeks, weight ± 200 grams. Rats were grouped into 6 groups with the first group (K1) as a control, the second group (K2) induced alloxan, the third group (K3) induced alloxan with glibenclamide, the fourth group (K4), the fifth group (K5), and the sixth group (K6) induced alloxan with green tea extract as the treatment with dose of green tea extract, 200 mg/kg, 400 mg/kg, and 800 mg/kg. The first, FBG level examination is done 3 days after induction of alloxan using a glucometer. Giving green tea extract was given for 16 days orally, then FBG level re-examined from the tail of rats. One Way ANOVA Test Results, there is the effectiveness of green tea extract on reducing FBG level (p = 0,000). The post hoc Bonferroni test showed that giving 800 mg/kg of green tea extract was the best dose in reducing KGDP close to normal (p = 0,000).
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References
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Ueda-Wakagi M, Nagayasu H, Yamashita Y, Ashida H. Green Tea Ameliorates Hyperglycemia by Promoting the Translocation of Glucose Transporter 4 in the Skeletal Muscle of Diabetic Rodents. International Journal Molecule Sciences [Internet]. 2019;20(10);2436. Available from:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566303/
Aryaeian N, Sedehi SK, Arablou T. Polyphenols and Their Effects on Diabetes Management: A Review. Medical Journal of The Islamic Republic of Iran (MJIRI) [Internet]. 2017;31(1):1-14. Available from: http://mjiri.iums.ac.ir/article-1-3843-en.pdf
Marella S. Flavonoids-The Most Potent Poly-phenols as Antidiabetic Agents: An Overview. Mod Appro Drug Des [Internet]. 2017;1(3):1–5. Available from: https://crimsonpublishers.com/madd/fulltext/MADD.000513.php
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Marks DB, Marks AD, Smith CM. Biokimia Kedokteran Dasar. Jakarta: EGC; 2012. p.285–418.
Namita P, Mukesh R, Vijay KJ. Camellia Sinensis (Green Tea): A Review. Global Journal of Pharmacology [Internet]. 2012;6(2):52–59. Available from: https://pdfs.semanticscholar.org/e55e/0b32e117c3a0ad1dc2277083c8d294f246c1.pdf
Park J, Bae J, Im S, & Song D. Green tea and type 2 diabetes. Integrative Medicine Research [Internet]. 2014;3:4–10. Available from: https://reader.elsevier.com/reader/sd/pii/S221342201300098X?token=8A65FAC5B33A0B15E0C9079E98542294ABB7899A33460A0DF4DFBC890852C410ACAA355C47FD96AECA17F81CDA8D03F6
Haidari F, Shahi MM, Zarei M, Rafiei H, Omidian K. Effect of Green Tea Extract on Body Weight, Serum Glucose and Lipid Profile in Streptozotocin-Induced Diabetic Rats: A Dose Response Study. Saudi Medicine Journal [Internet]. 2012;33(2):128–33. Available from: https://www.researchgate.net/publication/235637252_Effect_of_green_tea_extract_on_body_weight_serum_glucoseand_lipid_profile_in_streptozotocin-induced_diabetic_rats
Lahirin R, Permadhi I, Mudjihartini N, Rahmawati R, Sugianto R. Additional Benefit of Higher Dose Green Tea in Lowering Postprandial Blood Glucose. Medical Journal of Indonesia [Internet]. 2015;24(2):97–102. Available from: https://doi.org/10.13181/mji.v24i2.1167
Jin-Ming M, Shin-Yu C, Xin-Lin W, Ren-You G, Yuan-Feng W, Shu-Xian C, Xiao-Yu X, Pang-Zhen Z &Huan-Bin L. Effects and Mechanisms of Tea for the Prevention and Management of Diabetes Mellitus and Diabetic Complications: An Updated Review. Antioxidants [Internet]. 2019;8(6):1–25. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617012/
Kementrian Kesehatan RI (KEMENKES). Situasi dan Analisis Diabetes. Pusat Data dan Informasi Kementrian Kesehatan (KEMENKES) RI [Internet]. 2018; Available from: https://pusdatin.kemkes.go.id/download.php?file=download/pusdatin/infodatin/infodatin-Diabetes-2018.pdf.
Gardner DG, Shoback D. Greenspan’ S: Basic & Clinical Endocrinology. 10th Edition. New York: Mc Graw Hill Medical Book; 2018.
Centers of Disease Control (CDC). Diabetes and Prediabetes. National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP) [Internet]. 2019; Available from: https://www.cdc.gov/chronicdisease/resources/publications/factsheets/diabetes-prediabetes.htm
World Health Organization (WHO). Diabetes [Internet]. 2018; Available from: https://www.who.int/news-room/fact-sheets/detail/diabetes
Ighodaro OM, Adeosun AM, Akinloye OA. Alloxan-Induced Diabetes, A Common Model for Evaluating The Glycemic-Control Potential of Therapeutic Compounds and Plants Extracts in Experimental Studies. Medicina (Lithuania). Med [Internet]. 2017;53(6):365–74. Available from: https://doi.org/10.1016/j.medici.2018.02.001
Perkumpulan Endokrinologi Indonesia (PERKENI). Konsensus Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 di Indonesia 2015 [Internet]. Pengurus Besar Perkumpulan Endokrinologi Indonesia (PB PERKENI). 2015. p.1–16. Available from: https://pbperkeni.or.id/wp-content/uploads/2019/01/4.-Konsensus-Pengelolaan-dan-Pencegahan-Diabetes-melitus-tipe-2-di-Indonesia-PERKENI-2015.pdf
Simanjuntak HA. Pemanfaatan Tumbuhan Obat Diabetes Mellitus di Masyarakat Etnis Simalungun Kabupaten Simalungun Provinsi Sumatera Utara. Jurnal Biologi Lingkungan, Industri, Kesehatan [Internet]. 2018;5(1):59–71. Available from: http://dx.doi.org/10.31289/biolink.v5i1.1663
Qiu-Yue F, Qing-Sheng L, Xiao-Ming L, Ru-Ying Q, Rui Y, Xu-Min L, Zhan-Bo D, Li-Ping X, Xin-Qiang Z, Jian-Liang L, Cong-Bo Y, Jian-Hui Y & Yue-Rong L. Antidiabetic Effects of Tea. Molecules [Internet]. 2017;22(5):1–19. Available from: https://doi.org/10.3390/molecules22050849
Al Hroob AM, Abukhalil MH, Hussein OE, Mahmoud AM. Pathophysiological Mechanisms of Diabetic Cardiomyopathy And The Therapeutic Potential Of Epigallocatechin-3-Gallate. Biomedicine and Pharmacotherapy. [Internet]. 2019;109(2019):2155–72. Available from: https://doi.org/10.1016/j.biopha.2018.11.086
Takahashi M, Miyashita M, Suzuki K, Bae S, Kim H, Wakisaka T, Yasunaga K. Acute Ingestion of Catechin-Rich Green Tea Improves Postprandial Glucose Status and Increases Serum Thioredoxin Concentrations in Postmenopausal Women. British Journal of Nutrition. 2014;112(9):1542–50. Available from: https://doi:10.1017/S0007114514002530
Saraswati A. Efektivitas Ekstrak Daun Teh Hijau (Camellia sinensis) dengan NaOCL 2,5% terhadap Bakteri Enterococcus faecalis sebagai Alternatif Larutan Irigasi Saluran Akar. Repository Unhas [Internet]. 2015; Available from: http://repository.unhas.ac.id/handle/123456789/15669
Martono B, Setiyono RT. Skrining Fitokimia Enam Genotipe Teh. Jurnal Tanaman Industri dan Penyegar [Internet]. 2014;1(2):63-68. Available from: https://doi.org/10.21082/jtidp.v1n2.2014.p63-68
Rani R, Nagpal D, Gullaiya S, Madan S, Agrawal SS. Phytochemical, Pharmacological and Beneficial Effects of Green Tea. International Journal of Pharmacognosy and Phytochemical Research [Internet]. 2014;6(3):420–426. Available from: https://www.researchgate.net/publication/286210054_Phytochemical_pharmacological_and_beneficial_effects_of_green_tea
Swastini DA, Shaswati GA, Widnyana IP, Amin A, Kusuma L, Putra AA, Samirana PO. Penurunan Kadar Glukosa Darah dan Gambaran Histopatologi Pankreas dengan Pemberian Gula Aren (Arenga pinnata) pada Tikus Jantan Galur Wistar yang Diinduksi Aloksan. Indonesia Medicus Veterinus [Internet]. 2018;7(2):94–105. Available from: https://ojs.unud.ac.id/index.php/imv/article/view/39227/23718
Ueda-Wakagi M, Nagayasu H, Yamashita Y, Ashida H. Green Tea Ameliorates Hyperglycemia by Promoting the Translocation of Glucose Transporter 4 in the Skeletal Muscle of Diabetic Rodents. International Journal Molecule Sciences [Internet]. 2019;20(10);2436. Available from:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6566303/
Aryaeian N, Sedehi SK, Arablou T. Polyphenols and Their Effects on Diabetes Management: A Review. Medical Journal of The Islamic Republic of Iran (MJIRI) [Internet]. 2017;31(1):1-14. Available from: http://mjiri.iums.ac.ir/article-1-3843-en.pdf
Marella S. Flavonoids-The Most Potent Poly-phenols as Antidiabetic Agents: An Overview. Mod Appro Drug Des [Internet]. 2017;1(3):1–5. Available from: https://crimsonpublishers.com/madd/fulltext/MADD.000513.php
Kumari M, Jain S. Tannins: An Antinutrient with Positive Effect to Manage Diabetes. Research Journal of Recent Sciences [Internet]. 2012;1(12):70–73. Available from: https://pdfs.semanticscholar.org/042c/c6b1c232f7caded24a3713ed547f1f556f56.pdf
Nazaruk J, Borzym-Kluczyk M. The Role of Triterpenes in the Management of Diabetes Mellitus and Its Complications. Phytochemical Review [Internet]. 2015;14(4):675–90. Available from: http://dx.doi.org/10.1007/s11101-014-9369-x
Aboonabi A, Rahmat A, Othman F. Antioxidant Effect of Pomegranate Against Streptozotocin-Nicotinamide Generated Oxidative Stress Induced Diabetic Rats. Toxicology Reports [Internet]. 2014;1:915–922. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5598111/
Marks DB, Marks AD, Smith CM. Biokimia Kedokteran Dasar. Jakarta: EGC; 2012. p.285–418.
Namita P, Mukesh R, Vijay KJ. Camellia Sinensis (Green Tea): A Review. Global Journal of Pharmacology [Internet]. 2012;6(2):52–59. Available from: https://pdfs.semanticscholar.org/e55e/0b32e117c3a0ad1dc2277083c8d294f246c1.pdf
Park J, Bae J, Im S, & Song D. Green tea and type 2 diabetes. Integrative Medicine Research [Internet]. 2014;3:4–10. Available from: https://reader.elsevier.com/reader/sd/pii/S221342201300098X?token=8A65FAC5B33A0B15E0C9079E98542294ABB7899A33460A0DF4DFBC890852C410ACAA355C47FD96AECA17F81CDA8D03F6
Haidari F, Shahi MM, Zarei M, Rafiei H, Omidian K. Effect of Green Tea Extract on Body Weight, Serum Glucose and Lipid Profile in Streptozotocin-Induced Diabetic Rats: A Dose Response Study. Saudi Medicine Journal [Internet]. 2012;33(2):128–33. Available from: https://www.researchgate.net/publication/235637252_Effect_of_green_tea_extract_on_body_weight_serum_glucoseand_lipid_profile_in_streptozotocin-induced_diabetic_rats
Lahirin R, Permadhi I, Mudjihartini N, Rahmawati R, Sugianto R. Additional Benefit of Higher Dose Green Tea in Lowering Postprandial Blood Glucose. Medical Journal of Indonesia [Internet]. 2015;24(2):97–102. Available from: https://doi.org/10.13181/mji.v24i2.1167
Jin-Ming M, Shin-Yu C, Xin-Lin W, Ren-You G, Yuan-Feng W, Shu-Xian C, Xiao-Yu X, Pang-Zhen Z &Huan-Bin L. Effects and Mechanisms of Tea for the Prevention and Management of Diabetes Mellitus and Diabetic Complications: An Updated Review. Antioxidants [Internet]. 2019;8(6):1–25. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617012/
Published
2020-06-14
How to Cite
Hafshah, H., & Simanjuntak, K. (2020). Efektivitas Ekstrak Teh Hijau (Camellia sinensis L.) terhadap Penurunan Kadar Glukosa Darah Puasa Tikus Wistar (Rattus Norvegicus) yang di Induksi Aloksan. Jurnal Sehat Mandiri, 15(1), 86-97. https://doi.org/https://doi.org/10.33761/jsm.v15i1.194
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Articles
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